Dr. Hongwei Gai is a professor of School of Chemistry and Materials Science at Jiangsu Normal University since Dec. 2011. He earned his doctorate degree in analytical chemistry at Chinese Science of Academy, Dalian Institute of Chemical Physics in 2005. After he did his postdoctoral work in Health Science Center at Texas University in San Antonio, Dr. Gai joined Biomedical Engineering Center at Hunan University as an appointed professor in Dec. 2006. Dr. Gai became a full professor in Aug. 2009 and then in School of Biology after Jan. 2010.
Dr. Gai’s research focuses on single molecule fluorescence imaging and the application in chemistry and biology. Dr. Gai has published two books and over 60 scientific papers.
姓名：盖宏伟
学位学历：博士研究生
职称：教授
职务：实验室与设备管理处处长兼分析测试中心主任
学科专业：分析化学
E-mail：gai@jsnu.edu.cn
【个人简历】
1.教育背景和工作简历
1993年9月- 1997年7月:西安交通大学，化工学院，学士
1997年9月- 2000年7月:大连理工大学，化工学院，硕士
2000年9月- 2005年11月:中国科学院大连化学物理研究所，分析化学，博士
2005年11月－2006年10月：美国德州大学圣安东尼奥健康科学中心，博士后
2006年10月－2011年11月：湖南大学生物医学工程中心/生物学院，校聘教授/教授
2011年12月至今：江苏师范大学，化学与材料科学学院，教授
2013年4月-2018年7月：江苏师范大学化学与材料科学学院，副院长
2018.4月至今：江苏师范大学科学技术研究院，副院长
2021年12月至今：江苏师范大学，实验室与设备管理处，处长
2022年4月至今：江苏师范大学分析测试中心主任
2.工作成绩及学术成果。
已出版专著2部，发表SCI收录学术论文62篇，多数发表于Anal Chem， Lab Chip， Chem Commun， Angew Chem Int Ed等国际著名刊物。申请发明专利十余项，已授权9项。主持国家自然科学基金面上项目3项、青年项目1项，江苏省高校优秀科技创新团队负责人。第十六届徐州市政协委员。
【研究领域和兴趣】
主要从事单分子荧光检测和数字生物传感研究。以显微成像为主要手段，在细胞和单分子层面上，开展以高灵敏检测和动态监测为特征的疾病早期诊断和致病机理研究，包括数字免疫分析，单分子单颗粒传感，动态单分子间相互作用的监测，纳米显微技术等。
【代表论著】
专著：
1. Hongwei Gai, Qingquan Zhang, Xiaojun Liu, Chenghua Zong, Digital Immunoassays, DOI: 10.1201/9781003729211, ISBN: 9781003729211, CRC Press, 2026.1.
2.盖宏伟，张清泉等，《数字免疫分析》，ISBN 978-7-03-076032-6，北京：科学出版社，2023年8月
论文：
47. Xiaoxiao Yang, Shuangshuang Ma, Xiaoyan Pan, Qingquan Zhang*, Hongwei Gai*, Quantum dot digital immunoassays on a recyclable high-refractive-index bead array imaged with a low-numerical-aperture objective, Analytical Chemistry 2026, 98，6056-6064.
46. Ye Rong, Xiaowen Li, Jin Tian, Lei Bao, Dandan Zhao, Hao Chen*, Xiaojun Liu*, Hongwei Gai*, Homogeneous Digital Immunoassay for a Potential Noninvasive Urinary Biomarker of p62 for Neuronal Intranuclear Inclusion Disease, Analysis & Sensing 2026, 6, e202500222.
45. Baiyuan Zhao, Xiaoyan Pan, Qingquan Zhang*, Hongwei Gai*, Amplification-Free Dye-Labeled Digital Immunoassay: From Subpicomolar to Attomolar, Analytical Chemistry 2025, 97(31),16933-16941.
44. Chenghua Zong, Yafang Wang, Hongwei Gai*, A cascade signal amplification aptasensor based on the cis- and trans-cleavage activities of Cas12a for sensitive detection of thrombin, Microchemical Journal 2025, 213,113835.
43. Xiaojun Liu，Qingquan Zhang, Chenghua Zong, Hongwei Gai*, Digital Immunoassay for Proteins: Theory, Methodology, and Clinical Applications, Analytical Chemistry, 2025, 97,9077-9110.
42. Qingquan Zhang, Wenwen Chai, Xiaoyan Pan, Hongwei Gai *，Amplification-free digital immunoassay down to the attomolar level by synergistic sedimentation of Brownian motion suppression and dehydration transfer, Analytical Chemistry, 2024, 96,3054-3061.
41.ZihanXu, Xiaojun Liu, Chenghua Zong, Qingquan Zhang, Hongwei Gai*, Homogeneous immunoassay utilizing fluorescence resonance energy transfer from quantum dots to tyramide dyes deposited on full immunocomplexes, Analyst, 2023, 148, 4877-4884.
40. Xiaojun Liu, Xinyi Lin, Xiaoyan Pan, and Hongwei Gai*, Multiplexed Homogeneous Immunoassay Based on Counting Single Immunocomplexes together with Dark-Field and Fluorescence Microscopy, Analytical Chemistry, 2022, 94, 5830-5837.
39. 苏玉婷，盖宏伟*，单分子计数免疫分析，激光与光电子学进展，2022，59，0600018
38. Qingquan Zhang, Jiaqu Chen, Ziyin Zhong, Bowei Li*, Hongwei Gai*, Aqueous two-phase systems evolved double-layer film for enzymatic activity preservation: A universal protein storage strategy for paper based microdevice, Analytica Chimica Acta, 2022, 1197, 339540.
37. Qingquan Zhang, Jiajia Li, Xiaoyan Pan, Xiaojun Liu, Hongwei Gai*, Low-Numerical Aperture Microscope Objective Boosted by Liquid-Immersed Dielectric Microspheres for Quantum Dot-Based Digital Immunoassays, Analytical Chemistry, 2021, 93,12848-12853.
36. Qingquan Zhang, Jiajia Li, Yuting Su, Xiaoyan Pan, Hongwei Gai*, Ball-lens assisted sensitivity improvement of fluorescence immunoassay in microchannels, RSC Advances, 2021,11, 27541-27546.
35. Xiaojun Liu, Zhangjian Wu, Xinyi Lin, Wei Bu*, Lei Qin, Hongwei Gai*, Homogeneous Digital Biosensor for Circulating Tumor DNA by Enumerating Dual-color Quantum Dot Complex, Analyst, 2021, 11, 27541-27546.
34. Xiaojun Liu, Yuanyuan Sun, Xinyi Lin, Xiaoyan Pan, Zhangjian Wu, Hongwei Gai*, Digital duplex homogeneous immunoassay by counting immuno-complex labeled with quantum dots, Analytical Chemistry, 2021, 93, 3089-3095.
33. Qingquan Zhang, Xuebing Zhang, Jiajia Li, Hongwei Gai*, Non-stochastic protein counting analysis for precision biomarker detection: suppressing Poisson noise at ultralow concentration, Analytical Chemistry, 2020, Anal Chem 2020, 92, 654−658.
32. Qingquan Zhang, Jiaqu Chen, Hongwei Gai*, High-throughput-generating water-in-water droplet for monodisperse biocompatible particle synthesis, Journal of Materials Science, 2019, 54, 14905-14913.
31. Xiaojun Liu, Yusu Zhang, Aiye Liang, Hongwei Ding, Hongwei Gai*，Plasmonic resonance energy transfer from a Au nanosphere to quantum dots at a single particle level and its homogenous immunoassay, Chemical Communications, 2019, 55, 11442-11445.
30.Xiaojun Liu, Conghui Huang, Chenghua Zong, Aiye Liang, Zhangjian Wu, Yusu Zhang, Qingquan Zhang, Wenfeng Zhao, Hongwei Gai *,A Single-Molecule Homogeneous Immunoassay by Counting Spatially “Overlapping” Two-Color Quantum Dots with Wide-Field Fluorescence Microscopy, ACS Sensors, 2018,3, 2644-2650.
29.Xiaojun Liu, Conghui Huang, Xiuling Dong, Aiye Liang, Yusu Zhang, Qingquan Zhang, Qi Wang*, Hongwei Gai *, Asynchrony of spectral blue-shifts of quantum dot based digital homogeneous immunoassay, Chemical Communications, 2018, 54, 13103-13106.
28.Suli Dong, Xiaojun Liu, Qingquan Zhang, Wenfeng Zhao, Chenghua Zong, Aiye Liang, Hongwei Gai *, Sensing active heparin by counting aggregated quantum dots at single-particle level, ACS Sensors, 2017, 2, 80-86.
27. Qiangquan Zhang, Hanlin Li, Xiaojun Liu, Wenfeng Zhao, Chenghua Zong, Hongwei Gai *, A self-driven miniaturized liquid fuel cell, Chemical Communications, 2016, 52, 12068-12071.
26.Qingquan Zhang, Meng Xu, Xiaojun Liu, Wenfeng Zhao, Chenghua Zong, Yang Yu, Qi Wang *, Hongwei Gai*, Fabrication of Janus droplets by evaporation driven liquid-liquid phase separation, Chemical Communications, 2016, 52, 5015-5018.
25. Xiaojun Liu, Zhangjian Wu, Qingquan Zhang, Wenfeng Zhao, Chenghua Zong, Hongwei Gai*, Single Gold Nanoparticle-Based Colorimetric Detection of Picomolar Mercury Ion with Dark-Field Microscopy, Analytical Chemistry, 2016, 88, 2119-2124.
24. Wenfeng Zhao*, Wei Jia, Mimi Sun, Xiaojun Liu, Qingquan Zhang, Chenghua Zong, Hongwei Gai*, Colorimetric detection of Cu 2+ by surface coordination complexes of polyethyleneimine-capped Au nanoparticles, Sensors and Actuators: B. Chemical, 2016, 223, 411-416.
23. Xiaojun Liu, Yawei Wang, Yang Tu, Ziqi Zhu, Xiaogang Li, Qingquan Zhang, Wenfeng Zhao, Yuling Li, Hongwei Gai*, A rapid and simple approach for glycoform analysis, Analytica Chimica Acta, 2015, 865,71-75.
22. Xingbo Shi, Suli Dong, Minmin Li, Xiaojun Liu, Qingquan Zhang, Wenfeng Zhao, Chenghua Zong, Yewang Zhang, Hongwei Gai*, Counting quantum dot aggregates for the detection of biotinylated proteins, Chemical Communications, 2015, 51, 2353-2356.
21. Xingbo Shi, Minmin Li, Wenfeng Zhao, Aiye Liang , Xiaojun Liu, Hongwei Gai*, Spectral imaging superlocalization microscopy for quantum dots, Sensors and Actuators: B. Chemical, 2015, 207, 308-312.
20. Wenfeng Zhao, Suli Dong, Lichun Sun, Qi Wang*, Hongwei Gai*, Investigating the Photostability of Quantum Dots at the Single-MoleculeLevel, Chemistry-An Asian Journal, 2014, 9, 3542-3548.
19. Qingquan Zhang, Xiaojun Liu, Dayu Liu, Hongwei Gai*, Ultra small droplet generation via volatile component evaporation, Lab on a Chip, 2014,14(8), 1395 - 1400.
18. Xiaojun Liu, Qingquan Zhang, Yang Tu, Hongwei Gai*, Single Gold Nanoparticle Localized Surface Plasmon Resonance Spectral Imaging for Quantifying Binding Constant of Carbohydrate–Protein Interaction, Analytical Chemistry, 2013, 85, 11851–11857.
17. Xingbo Shi, Yang Tu, Xiaojun Liu, Edward S Yeung, Hongwei Gai*, Photobleaching of Quantum Dots by Non-resonant Light, Physical Chemistry Chemical Physics, 2013, 15, 3130-3132.
16. 刘晓君，涂洋，盖宏伟*，单分子宽场光学显微成像技术，化学进展，2013，25，370-379.
15. Xiaojun Liu, Hui Wang, Aiye Liang, Yuling Li, Hongwei Gai*, Bingcheng Lin*, Determination of binding constants between 1 one protein and multiple carbohydrates by affinity chromatography on a microchip, Journal of Chromatography A, 2012, 1270,340-343.
14. Xingbo Shi, Zhongqiu Xie, Yuehong Song, Yongjun Tan, Edward S. Yeung, Hongwei Gai*, Super-Localization Spectral Imaging Microscopy of Multi-color Quantum Dot Complex, Analytical Chemistry , 2012, 84(3),1504-1509.
13. Linfen Yu, Qun Li, Hongwei Gai*, Zhanhui Wang*, Chemiluminescence Response of Murine Macrophages on Multilayer Microfluidic Chips, Appl. Biochem. Biotechnol., 2012, 166, 786-795.
12. Hongwei Gai*, Yongjun Li*, Edward S Yeung, Optical detection system of microfluidic chip, Topics in Current Chemistry，2011，304:171-201
11. Xingbo Shi, Xianxin Meng, Lichun Sun, Jinhua Liu, Jing Zheng, Hongwei Gai*, Ronghua Yang, Edward S Yeung, Observing photophysical properties of quantum dots in air at the single molecule level: advantages in microarray applications, Lab on a Chip, 2010, 10, 2844-2847. (Outside front cover)
10. Xiaobing Bu, Huaping Chen, Hongwei Gai*, Ronghuang Yang, Edward S Yeung, Scattering imaging of single quantum dots with dark-field microscopy, Analytical Chemistry, 2009, 81, 7507-7509.
9. Nan Li, Hui Tang, Hongwei Gai,* Xiuling Dong, Qi Wang, Edward S Yeung, Determination of protein surface excess on a liquid/solid interface by single-molecule counting, Analytical and Bioanalytical Chemistry, 2009, 394, 1879-1885.
8. Huaping Chen, Hongwei Gai,* Edward S Yeung, Inhibition of Photobleaching and Blue Shift in Quantum Dots, Chemical Communications, 2009, 1676-1678.
7. Rui Han, Yewang Zhang, Xiuling Dong, Hongwei Gai*, Edward Yeung, Spectral imaging of single molecules by transmission grating-based epi-fluorescence microscopy, Analytica Chimica Acta, 2008, 619, 209-214.
6. Qun Li, Rui Han, Xianxin Meng, Hongwei Gai*, Edward Yeung, Tracking single quantum dot and its spectrum in free solution with controllable thermal diffusion suppression, Analytical Biochemistry, 2008, 377 , 176–181
5. Hongwei Gai, Isaac Stayton, Xin Liu, Bingcheng Lin, Yinfa Ma, Visualizing chemical interactions in life sciences with wide-field fluorescence microscopy towards the single-molecule level, Trends in Analytical Chemistry, 2007, 26, 980-992.
4. Hongwei Gai, Gary A. Griess, Borries Demeler, Susan T. Weintraub, Philip Serwer, Routine fluorescence microscopy of single untethered protein molecules confined to a planar zone, Journal of Microscopy, 2007, 226, 256–262.
3. Hongwei Gai, Qi Wang, Yinfa Ma, Bingcheng Lin, Correlations between molecular numbers and molecular masses in an evanescent field and their applications in probing molecular interactions. Angewandte Chemie International Edition, 2005, 44, 5107-5110.
2. Hongwei Gai, Ying Li, Zhan-Hua Silber-Li, Yinfa Ma, Bingcheng Lin, Simultaneously measurements of bulk flow and near wall flow velocity in a microchannel at single molecule level, Lab on a Chip, 2005, 5, 443-449.
1. Hongwei Gai, Linfen Yu, Zhongpeng Dai, Yinfa Ma, Bingcheng Lin., Injection by Hydrostatic Pressure Conjunction with Electrokinetic Force on a Microfluidic Chip, Electrophoresis, 2004, 25,1888-1894.
